L. Tracy, L. Cabuang, R. McCaw & R. Hammond
(Updated September 2006)
The Molecular Microbiology Unit at VIDRL has performed HCV genotyping
since 1994. We now have a comprehensive database of results from
more than 4000 patients. The commercial kit we use is the Versant
HCV Genotyping Assay (LiPA) from Bayer. The region used to identify
the genotypes in this assay is the 5’ UTR. It must be noted
that recombination events are possible and that in these cases,
using only one region may not be sufficient to determine HCV genotype
The interpretation of the significant bands is dependent on their
relative intensity and is by its very nature, subjective. With
experience, the significant bands are recognized, with the bands
for some genotypes being clearer than others. For example, we
have found that the bands for genotype 3a are mostly clear, whereas
those for genotype 1 subtypes often have faint secondary bands.
As a resource for laboratories using the Versant HCV Genotype
Assay (LiPA) we have compiled a set of examples with characteristic
banding patterns not represented on the interpretation chart supplied
with the kit. It is possible that there are regional differences
in genotype sequence, so that the interpretation chart may not
be universally suitable. We have sequenced the core region from
at least three samples with the same unusual banding pattern before
assigning the genotype and placing it on our website.
To begin your search, please click here for the Unusual
Banding Interpretation Chart.
Note: Brackets - () - denote faint bands.
Before printing please ensure your page setup is landscape.
Classification changes:
Recently, there has been a review of the nomenclature for HCV
genotypes. Here is a table outlining the new codes, replacing
the previous codes for these genotypes.
|
New code |
3k |
6c |
6d |
6e |
6f |
6g |
6h |
6i |
6j |
6k |
6l |
Old code |
10a |
7d |
7b |
7a |
7c |
11a |
9a |
9b |
9c |
8b |
8a |
