Mycobacterial identification and sensitivity testing on a referred pure isolate

Test Method
Identification- Phenotypic & molecular depending on isolate identification. Various culture methods for drug susceptibility testing
Specimen Required
Slope, Solid or broth culture media.
When Tested
Daily
Turnaround Time
Dependant on growth on subculture
Normal Ranges
n/a
Reference Ranges
n/a
Cut Off Lower
n/a
Units of Measurement
n/a
Related Tests
See text (MRL section) for further details
Billing Advice
Reference function, not billed.
Contact
9342 9617

Mycobacterium leprae PCR

Test Method
Real-time PCR
Specimen Required
Fresh or paraffin embedded fixed tissue, nasal secretions, swabs
When Tested
On request
Normal Ranges
NA
Reference Ranges
Ct <40
Cut Off Lower
Ct value
Units of Measurement
Ct
Contact
9342 9617

Mycobacterium tuberculosis complex PCR

Test Method
Real-time PCR
Specimen Required
Respiratory specimens, biopsies, whole CSF (>1ml). swabs, Paraffin embedded tissue sections (at least 6 sections, each 10-20 um thick)
Spec Type Min
1 ml for CSF
When Tested
3x weekly
Turnaround Time
1-2 working days
Normal Ranges
NA
Reference Ranges
Ct <40
Cut Off Lower
Ct value
Units of Measurement
Ct
Related Tests
See text (MRL section) for further details. Contact laboratory for suitability of specimens. Current method: TaqMan real-time PCR (IS6110 – based target).
Billing Advice
Non-rebatable
Contact
9342 9617

Mycobacterium tuberculosis/RIF GeneXpert assay

Test Method
Real-time PCR
Specimen Required
Sputum
Spec Type Min
0.5 ml of respiratory specimen
When Tested
On request
Turnaround Time
1 day
Normal Ranges
NA
Reference Ranges
Ct value <36
Cut Off Lower
NA
Units of Measurement
Ct
Related Tests
RPOBSEQ TBPCR
Contact
9342 9617

Mycobacterium tuberculosis/RIF GeneXpert assay

Test Method
Real-time PCR
Specimen Required
Respiratory specimens, tissues (fresh and paraffin-embedded fixed tissue), body fluids, CSF, M. tuberculosis cultures (broth and solid media)
Spec Type Min
0.5 ml of respiratory specimen
When Tested
On request
Turnaround Time
1 day
Normal Ranges
NA
Reference Ranges
Ct value <36
Cut Off Lower
NA
Units of Measurement
Ct
Related Tests
RPOBSEQ TBPCR
Contact
9342 9617

Mycobacterium ulcerans PCR

Test Method
Real-time PCR
Specimen Required
Fresh tissue biopsy, swabs and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 um thick)
When Tested
3x weekly
Turnaround Time
1-2 working days
Normal Ranges
n/a
Reference Ranges
Ct value <35
Cut Off Lower
Ct value
Units of Measurement
Ct
Related Tests
n/a
Billing Advice
Non-rebatable
Contact
9342 9617

Mycoplasma genitalium PCR

Test Method
Anyplex STI-7 Real Time PCR
Specimen Required
Genital swab, first-pass urine.
Spec Type Min
n/a
When Tested
2x Weekly
Turnaround Time
2x Weekly
Normal Ranges
n/a
Reference Ranges
n/a
Cut Off Lower
n/a
Units of Measurement
Cyclic catcher melting temperature (Cyclic CMTA)
Billing Advice
Medicare rebatable
Contact
9342 9670

Mycoplasma pneumoniae PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Any respiratory sample, CSF.
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Mycoplasma pneumoniae Serology

Test Method
Total Ab PAGL ,IgM EIA
Specimen Required
Serum (clotted blood)
When Tested
2x Weekly for Total Ab and IgM
Reference Ranges
total Ab
titre <40 neg
titre >40 pos
titre = 40 equiv
IgM
pos >cutoff value
neg equiv +/- 10%
Units of Measurement
PAGL- TITRE, IgM Optical density
Contact
9342 9644