Hepatitis C virus Serology – Supplementary

Test Method
ECLIA
Specimen Required
Serum, Cadaver, Plasma
Spec Type Min
300ul
When Tested
3 x Weekly
Reference Ranges
Reactive >= 1,Equiv 0.9-0.99, Non-Reactive < 0.9
Units of Measurement
COI
Contact
9342 9647 / 9342 9648

Hepatitis D virus PCR

Test Method
In-house conventional RT-PCR; positive sample quantified by real-time PCR
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
500 uL
When Tested
Weekly
Turnaround Time
2 Weeks.
Reference Ranges
375-10,000,000 IU/ml
Cut Off Lower
375 IU/mL
Units of Measurement
IU/mL
Contact
9342 9615

Hepatitis D virus Serology (Ab)

Test Method
Antibody CLIA
Specimen Required
Serum (clotted blood)
Spec Type Min
300ul
When Tested
Daily
Reference Ranges
Cut off = (0.5 x mean NC) + (0.5 x mean PC); =cut off = Reactive; > cut off = Non-Reavtive
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Hepatitis E virus Genotyping

Test Method
Conventional PCR; Capillary Sequencing
Specimen Required
Serum (Clotted Blood), Plasma (Not Heparin)
Spec Type Min
500uL
When Tested
As requested
Turnaround Time
Three Weeks
Normal Ranges
Not Applicable
Reference Ranges
N/A
Cut Off Lower
1000 IU/ml
Units of Measurement
N/A
Contact
9342 9615

Hepatitis E virus PCR

Test Method
Commercial assay (Altona)
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
500 uL
When Tested
On request
Turnaround Time
N/A
Normal Ranges
Not Applicable
Reference Ranges
LLOQ 200 IU/mL
Cut Off Lower
200 IU/ml
Units of Measurement
IU/ml
Related Tests
Hepatitis E Virus Genotyping
Contact
9342 9615

Hepatitis E virus Serology

Test Method
IgG EIA
Specimen Required
Serum (clotted blood), Plasma
Spec Type Min
100ul
When Tested
Weekly
Reference Ranges
cutoff value = 0.5 + neg control mean
neg pos >cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
S/CO,
Contact
9342 9647 / 9342 9648

Herpes simplex virus (HSV) Phenotyping for drug resistance

Test Method
HSV PCR screen followed by Virus isolation & drug susceptibility
Specimen Required
HSV isolate or specimen for HSV isolation
Spec Type Min
200uL
When Tested
On request
Turnaround Time
>7 days
Reference Ranges
n/a
Related Tests
HSV drug resistance genotype
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus genotyping

Test Method
Conventional PCR
Specimen Required
HSV positive sample
Spec Type Min
200uL
When Tested
On request
Turnaround Time
7 Days
Reference Ranges
n/a
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus Serology (HSV)

Test Method
HSV IgG, HSV IgM, HSV-1 IgG, HSV-2 IgG EIA
Specimen Required
Serum, CSF, Plasma
Spec Type Min
300ul
When Tested
2x Weekly
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV)

Test Method
HSV IgG, HSV IgM, HSV-1 IgG, HSV-2 IgG EIA
Specimen Required
CSF
Spec Type Min
300ul
When Tested
2x Weekly
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental)

Test Method
Antibody / Antigen.
Specimen Required
Whole blood, serum or plasma
Reference Ranges
Positive HIV-1 = Any 2 bands of the 4 HIV-1 test lines with at least 1 ENV gp160 (band 4) or gp41 (band 6), Negative = no band, Indeterminate = 1 ENV (band 4 or 6), 1 GAG (band 5), 1 POL (band 3) or 1 GAG and 1 POL (bands 5 and 3). Positive HIV-2 = 2 HIV-2 bands gp36 and gp140 (band 1 and 2), Negative = no band, Indeterminate = 1 ENV gp36 (band 1) or gp140 (band 2), gp36 (band 1) alone or gp140 (band 2) alone.
Contact
9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental)

Test Method
Antibody / Antigen.
Specimen Required
CSF
Reference Ranges
Positive HIV-1 = Any 2 bnads of the 4 HIV-1 test lines with at least 1 ENV gp160 (band 4) or gp41 (band 6), Negative = no band, Indeterminate = 1 ENV (band 4 or 6), 1 GAG (band 5), 1 POL (band 3) or 1 GAG and 1 POL (bands 5 and 3). Positive HIV-2 = 2 HIV-2 bands gp36 and gp140 (band 1 and 2), Negative = no band, Indetermiante = 1 ENV gp36 (band 1) or gp140 (band 2), gp36 (band 1) alone or gp140 (band 2) alone.
Contact
9342 9647 / 9342 9648

HIV-2 Drug Resistance Genotyping (Protease and RT regions)

Test Method
Conventional PCR , sequencing and analysis
Specimen Required
ACD/EDTA blood
Spec Type Min
2ml of whole blood or 500ul of plasma
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
Non-rebatable
Contact
9342 9625

HIV-2 Proviral DNA Detection by Real Time PCR

Test Method
Real-time PCR (Taqman)
Specimen Required
ACD/EDTA blood
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
10 days
Reference Ranges
validation in progress for limitations
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625

Human herpesvirus 6 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (ACD or EDTA), CSF, swab, tissue
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Quantitation

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (EDTA or ACD), plasma
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
DNA Copies/mL
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Serology

Test Method
IgG IFA, IgM IFA
Specimen Required
Serum (clotted blood)
When Tested
Weekly
Reference Ranges
IgG
titre >20 pos,
titre <20 neg,
titre 20 equiv;
IgM
titre <10 not detected,
titre >10 detected
Units of Measurement
TITRE
Contact
9342 9647 / 9342 9648

Human herpesvirus 7 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (ACD or EDTA), CSF
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 8 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (EDTA)
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human immunodeficiency virus – 1 proviral DNA tropism genotyping

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
ACD or EDTA
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
not detected (virus load)
Billing Advice
ViiV healthcare
Contact
9342 9625

Human immunodeficiency virus – 2 RNA Quantitation

Test Method
Real-time Taqman based PCR
Specimen Required
Blood (ACD or EDTA)
Spec Type Min
500ul of plasma or 2ml of whole blood
When Tested
On request
Turnaround Time
5 days
Reference Ranges
detectable from 500 copies/ml
Cut Off Lower
Target <500 copies/ml can be detected but not quantified
Units of Measurement
HIV-2 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625

Human Immunodeficiency virus Co-Receptor Tropism Genotype

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD or EDTA), plasma, serum and CSF
Spec Type Min
500ul of plasma, serum or CSF (RNA tropism)
When Tested
Weekly
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
ViiV healthcare
Contact
9342 9625

Human immunodeficiency virus DNA Detection by Real-Time PCR

Test Method
Real-time PCR (Taqman)
Specimen Required
ACD/EDTA blood
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
5 days
Reference Ranges
validation in progress for limitations
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625