Handbook Tests Archive | Page 8 of 10

Mycoplasma/Ureaplasma species PCR

Specimen Required: Swab, Urine
When Tested: 2x Weekly
Turnaround Time: 2x Weekly
Units of Measurement: Cyclic catcher melting temperature (Cyclic CMTA)
Contact: 9342 9670

Nipah virus PCR (Henipah combo assay)

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: 3-45 Ct
Contact: 9342 9628

Norovirus Genotyping

Test Method: Sanger Sequencing
Specimen Required: Faeces, Nucleic Acid Extract, Vomit
When Tested: On Request
Turnaround Time: 2 Weeks
Reference Ranges: n/a
Contact: 9342 9628

Norovirus Real Time PCR – RIDAGENE

Test Method: real time RT-PCR
Specimen Required: Bowel Contents, Faeces, Vomit
When Tested: 2x Weekly
Turnaround Time: 7 Days
Reference Ranges: n/a
Contact: 9342 9628

Orf virus PCR (see Poxvirus PCR)

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: 3-45 Ct
Contact: 9342 9628

Parainfluenza virus detection

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Turnaround Time: same day / next day
Reference Ranges: 3-45 Ct
Related Tests: see Respiratory virus PCR
Billing Advice: Non-rebatable
Contact: 9342 9628

Parasite Identification (ecto- or endo-parasites)

Test Method: Staining and microscopy
Specimen Required: Aspirate, Blood (Gel Tube), Blood (Whole), Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Nail Clippings, Serum, Sputum, Swab, Tissue, Urine, Washings
When Tested: On request
Turnaround Time: 7 days
Billing Advice: Non-rebatable
Contact: 9342 9670

Parechovirus PCR

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Turnaround Time: same day / next day
Reference Ranges: 3-45 Ct
Units of Measurement: Ct
Related Tests: See Enterovirus PCR
Billing Advice: Non-rebatable
Contact: 9342 9628

Parechovirus PCR genotyping (subsequent testing attempts to genotype the enterovirus using a different gene target)

Test Method: conventional PCR, sequencing, analysis
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Turnaround Time: 1 Month
Reference Ranges: n/a
Contact: 9342 9628

Parvovirus B19 PCR

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Turnaround Time: same day/ next day
Reference Ranges: 3-45 Ct
Units of Measurement: Ct
Billing Advice: Non-rebatable
Contact: 9342 9628

Parvovirus B19 Serology

Test Method: IgG, IgM EIA
Specimen Required: Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (Sodium Citrate), Serum
When Tested: 2x Weekly
Reference Ranges: cutoff control mean = cutoff value
neg pos >cutoff x 1.15
cutoff x 0.85 < equiv < cutoff value x 1.15
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

Picornavirus(see Respiratory PCR)

Test Method: Conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: n/a
Contact: 9342 9628

Pneumocystis jiroveci (carinii) PCR

Test Method: Taqman real time PCR Assay
Specimen Required: Aspirate, Biopsy, Cerebrospinal Fluid, Fluid, Lavage, Plasma, Sputum, Swab, Tissue, Washings, Serum
Spec Type Min: 200uL
When Tested: daily (on request)
Turnaround Time: 24 hour
Normal Ranges: n/a
Reference Ranges: Ct <32 - positive
Ct>32-40 - low positive
Cut Off Lower: Positive: 32 ct-value, Low positive >/=33 ct value
Units of Measurement: Cycle Theshold (Ct-value)
Related Tests: n/a
Billing Advice: Medicare rebatable
Contact: 9342 9670

Poliovirus 1, 2, 3 Serology

Test Method: Virus neutralisaion
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min: 500uL
When Tested: Contact laboratory
Turnaround Time: Two weeks once acute and convalescent sera are received
Normal Ranges: N/A
Reference Ranges: N/A
Cut Off Lower: N/A
Units of Measurement: N/A
Related Tests: Poliovirus isolation
Billing Advice: N/A
Contact: 9342 9607

Poliovirus Intratypic Differentiation and Vaccine Derived Poliovirus

Test Method: Real Time RT-PCR
Specimen Required: Culture Isolate, Nucleic Acid Extract, Virus Isolate
Spec Type Min: 50 ul cell culture lysate
When Tested: On Request
Turnaround Time: 7 Days
Normal Ranges: N/A
Reference Ranges: N/A
Cut Off Lower: N/A
Units of Measurement: Ct
Related Tests: Poliovirus isolation, Poliovirus sequencing, Enterovirus typing
Billing Advice: N/A
Contact: 9342 9607

Poliovirus Sequencing

Test Method: RT-PCR, Nucleic acid sequencing
Specimen Required: Culture Isolate, Faeces, Nucleic Acid Extract, Virus Isolate
Spec Type Min: 20 ul nucleic acid extract, 50 ul cell culture lysate or specimen extract
When Tested: On Request
Turnaround Time: 7 Days
Normal Ranges: N/A
Reference Ranges: N/A
Cut Off Lower: N/A
Units of Measurement: N/A
Related Tests: Enterovirus (poliovirus) Isolation, Poliovirus Intratypic Differentiation and Vaccine Derived Poliovirus, Enterovirus Typing
Billing Advice: N/A
Contact: 9342 9607

Poxvirus PCR

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On request
Turnaround Time: same day / next day
Reference Ranges: 3-45 Ct
Units of Measurement: Ct
Billing Advice: Non-rebatable
Contact: 9342 9628

Q Fever (Coxiella burneti) Phase 2 Serology, (pre-vaccination screen)

Test Method: IgG EIA
Specimen Required: Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
When Tested: 2x Weekly, IgM x2 weekly
Reference Ranges: IgG
upper cutoff = Std value x factor (batch specific)
lower cutoff = Std value x factor (batch specific)
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

QuantiFERON ® -TB Gold Plus In-Tube Assay

Test Method: EIA
Specimen Required: Blood (Lithium Heparin)
Spec Type Min: exactly 1.0 mL blood per tube.
When Tested: On Request
Turnaround Time: 72 hours
Normal Ranges: n/a
Reference Ranges: > 0.35 units
Cut Off Lower: 0.35 for antigen
Units of Measurement: IU g-IFN
Related Tests: earlier quantiferon versions, mantoux test.
Billing Advice: Non-rebatable
Contact: 9342 9670

Rabies virus genus PCR

Test Method: Conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Nucleic Acid Extract, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: n/a
Contact: 9342 9628

Rabies virus PCR(including Australian Bat Lyssa virus)

Test Method: Conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Nucleic Acid Extract, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: n/a
Contact: 9342 9628

Respiratory Syncytial virus PCR

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Turnaround Time: same day / next day
Related Tests: see Respiratory virus PCR
Billing Advice: Non-rebatable
Contact: 9342 9628

Respiratory virus PCR (including Infuenza A, B, C; Parainfluenza 1, 2, 3; Coronaviruses; Adenoviruses; Human Metapneumovirus; Respiratory Syncytial virus (RSV); Picornaviruses)

Test Method: Real-time TaqMan PCR, conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Reference Ranges: 3-45 Ct
Contact: 9342 9628

Rift Valley fever virus PCR

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On request
Turnaround Time: same day / next day
Reference Ranges: 3-45 Ct
Units of Measurement: Ct
Billing Advice: Non-rebatable
Contact: 9342 9628

Ross River virus PCR

Test Method: Conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On Request
Contact: 9342 9628

Victorian Infectious Diseases Reference Laboratory

Mycoplasma/Ureaplasma species PCR

Mycoplasma/Ureaplasma species PCR

Nipah virus PCR (Henipah combo assay)

Norovirus Genotyping

Norovirus Real Time PCR – RIDAGENE

Orf virus PCR (see Poxvirus PCR)

Parainfluenza virus detection

Parasite Identification (ecto- or endo-parasites)

Parechovirus PCR

Parechovirus PCR genotyping (subsequent testing attempts to genotype the enterovirus using a different gene target)

Parvovirus B19 PCR

Parvovirus B19 Serology

Picornavirus(see Respiratory PCR)

Pneumocystis jiroveci (carinii) PCR

Poliovirus 1, 2, 3 Serology

Poliovirus Intratypic Differentiation and Vaccine Derived Poliovirus

Poliovirus Sequencing

Poxvirus PCR

Q Fever (Coxiella burneti) Phase 2 Serology, (pre-vaccination screen)

QuantiFERON ® -TB Gold Plus In-Tube Assay

Rabies virus genus PCR

Rabies virus PCR(including Australian Bat Lyssa virus)

Respiratory Syncytial virus PCR

Respiratory virus PCR (including Infuenza A, B, C; Parainfluenza 1, 2, 3; Coronaviruses; Adenoviruses; Human Metapneumovirus; Respiratory Syncytial virus (RSV); Picornaviruses)

Rift Valley fever virus PCR

Ross River virus PCR

Index