Hepatitis C virus PCR

Test Method
RT-PCR with hybridization detection or real-time TaqMan PCR, COBAS 6800
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
1 ml
When Tested
weekly
Turnaround Time
6 days
Normal Ranges
N/A
Reference Ranges
LLOD 15 IU/ml
Cut Off Lower
15 IU/ml
Units of Measurement
IU/ml
Contact
9342 9615

Hepatitis C virus Quantification

Test Method
Real-time TaqMan PCR
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
1 ml
When Tested
Twice weekly
Turnaround Time
6 Days.
Normal Ranges
HCVABB = 12 IU to 100,000,000 IU/ml. HCVABT = 12 IU to 100,000,000 IU/ml.
Reference Ranges
12 - 100 million IU/ml
Cut Off Lower
HCVABT = 12 IU/ml.
Units of Measurement
IU/ml
Contact
9342 9615

Hepatitis C virus Serology

Test Method
EIA
Specimen Required
Serum, Cadaver, Plasma
Spec Type Min
300ul
When Tested
Daily
Reference Ranges
Murex EIA (screening test)
cutoff formula;
neg control + 0.6
index formula; S/cutoff
index <0.9 = neg
index ³1.1 = pos
index 0.9-1.0 = equiv
Biorad EIA (supplemental test)
cutoff = pos control mean/5
neg pos >cutoff value
equiv +/- 10% cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Hepatitis C virus Serology – Supplementary

Test Method
ECLIA
Specimen Required
Serum, Cadaver, Plasma
Spec Type Min
300ul
When Tested
3 x Weekly
Reference Ranges
Reactive >= 1,Equiv 0.9-0.99, Non-Reactive < 0.9
Units of Measurement
COI
Contact
9342 9647 / 9342 9648

Hepatitis D virus PCR

Test Method
In-house conventional RT-PCR; positive sample quantified by real-time PCR
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
500 uL
When Tested
Weekly
Turnaround Time
2 Weeks.
Reference Ranges
1000 IU/ml
Cut Off Lower
1,000 copies/ml
Units of Measurement
copies/ml
Contact
9342 9615

Hepatitis D virus Serology (Ab)

Test Method
Antibody CLIA
Specimen Required
Serum (clotted blood)
Spec Type Min
300ul
When Tested
Daily
Reference Ranges
Cut off = (0.5 x mean NC) + (0.5 x mean PC); =cut off = Reactive; > cut off = Non-Reavtive
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Hepatitis E virus Genotyping

Test Method
Conventional PCR; Capillary Sequencing
Specimen Required
Serum (Clotted Blood), Plasma (Not Heparin)
Spec Type Min
500uL
When Tested
As requested
Turnaround Time
Three Weeks
Normal Ranges
Not Applicable
Reference Ranges
N/A
Cut Off Lower
1000 IU/ml
Units of Measurement
N/A
Contact
9342 9615

Hepatitis E virus PCR

Test Method
Commercial assay (Altona)
Specimen Required
Serum (clotted blood), Blood (ACD or EDTA)
Spec Type Min
500 uL
When Tested
On request
Turnaround Time
N/A
Normal Ranges
Not Applicable
Reference Ranges
LLOQ 500 copies/ml
Cut Off Lower
500 IU/ml
Units of Measurement
IU/ml
Related Tests
Hepatitis E Virus Genotyping
Contact
9342 9615

Hepatitis E virus Serology

Test Method
IgG EIA
Specimen Required
Serum (clotted blood), Plasma
Spec Type Min
100ul
When Tested
Weekly
Reference Ranges
cutoff value = 0.5 + neg control mean
neg pos >cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
S/CO,
Contact
9342 9647 / 9342 9648

Herpes simplex virus (HSV) Phenotyping for drug resistance

Test Method
HSV PCR screen followed by Virus isolation & drug susceptibility
Specimen Required
HSV isolate or specimen for HSV isolation
Spec Type Min
200uL
When Tested
On request
Turnaround Time
>7 days
Reference Ranges
n/a
Related Tests
HSV drug resistance genotype
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus genotyping

Test Method
Conventional PCR
Specimen Required
HSV positive sample
Spec Type Min
200uL
When Tested
On request
Turnaround Time
7 Days
Reference Ranges
n/a
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus Serology (HSV)

Test Method
HSV IgG, HSV IgM, HSV-1 IgG, HSV-2 IgG EIA
Specimen Required
Serum, CSF, Plasma
Spec Type Min
300ul
When Tested
2x Weekly
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV)

Test Method
HSV IgG, HSV IgM, HSV-1 IgG, HSV-2 IgG EIA
Specimen Required
CSF
Spec Type Min
300ul
When Tested
2x Weekly
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental)

Test Method
Antibody / Antigen.
Specimen Required
Whole blood, serum or plasma
Reference Ranges
Positive HIV-1 = Any 2 bands of the 4 HIV-1 test lines with at least 1 ENV gp160 (band 4) or gp41 (band 6), Negative = no band, Indeterminate = 1 ENV (band 4 or 6), 1 GAG (band 5), 1 POL (band 3) or 1 GAG and 1 POL (bands 5 and 3). Positive HIV-2 = 2 HIV-2 bands gp36 and gp140 (band 1 and 2), Negative = no band, Indeterminate = 1 ENV gp36 (band 1) or gp140 (band 2), gp36 (band 1) alone or gp140 (band 2) alone.
Contact
9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental)

Test Method
Antibody / Antigen.
Specimen Required
CSF
Reference Ranges
Positive HIV-1 = Any 2 bnads of the 4 HIV-1 test lines with at least 1 ENV gp160 (band 4) or gp41 (band 6), Negative = no band, Indeterminate = 1 ENV (band 4 or 6), 1 GAG (band 5), 1 POL (band 3) or 1 GAG and 1 POL (bands 5 and 3). Positive HIV-2 = 2 HIV-2 bands gp36 and gp140 (band 1 and 2), Negative = no band, Indetermiante = 1 ENV gp36 (band 1) or gp140 (band 2), gp36 (band 1) alone or gp140 (band 2) alone.
Contact
9342 9647 / 9342 9648

HIV-2 Drug Resistance Genotyping (Protease and RT regions)

Test Method
Conventional PCR , sequencing and analysis
Specimen Required
ACD/EDTA blood
Spec Type Min
2ml of whole blood or 500ul of plasma
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
Non-rebatable
Contact
9342 9625

HIV-2 Proviral DNA Detection by Real Time PCR

Test Method
Real-time PCR (Taqman)
Specimen Required
ACD/EDTA blood
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
10 days
Reference Ranges
validation in progress for limitations
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625

Human herpesvirus 6 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (ACD or EDTA), CSF, swab, tissue
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Quantitation

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (EDTA or ACD), plasma
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
DNA Copies/mL
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Serology

Test Method
IgG IFA, IgM IFA
Specimen Required
Serum (clotted blood)
When Tested
Weekly
Reference Ranges
IgG
titre >20 pos,
titre <20 neg,
titre 20 equiv;
IgM
titre <10 not detected,
titre >10 detected
Units of Measurement
TITRE
Contact
9342 9647 / 9342 9648

Human herpesvirus 7 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (ACD or EDTA), CSF
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 8 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Blood (EDTA)
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human immunodeficiency virus – 1 proviral DNA tropism genotyping

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
ACD or EDTA
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
not detected (virus load)
Billing Advice
ViiV healthcare
Contact
9342 9625