Human immunodeficiency virus – 1 proviral DNA tropism genotyping

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
not detected (virus load)
Billing Advice
ViiV healthcare
Contact
9342 9625

Human immunodeficiency virus – 2 RNA Quantitation

Test Method
Real-time Taqman based PCR
Specimen Required
Plasma (ACD), Plasma (EDTA)
Spec Type Min
500ul of plasma or 2ml of whole blood
When Tested
On request
Turnaround Time
5 days
Reference Ranges
detectable from 500 copies/ml
Cut Off Lower
Target <500 copies/ml can be detected but not quantified
Units of Measurement
HIV-2 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625

Human Immunodeficiency virus Co-Receptor Tropism Genotype

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Buffy Coat, Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
500ul of plasma, serum or CSF (RNA tropism)
When Tested
Weekly
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
ViiV healthcare
Contact
9342 9625

Human immunodeficiency virus DNA Detection by Real-Time PCR

Test Method
Real-time PCR (Taqman)
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Whole), Buffy Coat
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
5 days
Reference Ranges
validation in progress for limitations
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625

Human immunodeficiency virus Drug Resistance Mutations (Fusion Inhibitors)

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD), Blood (EDTA), Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
500ul of plasma or 2ml of whole blood
When Tested
On Request
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625

Human immunodeficiency virus Genotyping for drug resistance – Integrase

Test Method
Conventional PCR, sequencing and analysis, Next Gen Sequencing
Specimen Required
Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
2mL Whole Blood, 500uL or Plasma/Serum/CSF
When Tested
Weekly
Turnaround Time
2-4 Weeks
Cut Off Lower
Sensitivity of Assay = 500 RNA copies/mL
Billing Advice
Medciare Rebateable
Contact
9342 9625

Human immunodeficiency virus Genotyping for drug resistance – protease & reverse transcriptase

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD), Blood (EDTA), Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
2ml of whole blood or 500ul of plasma, serum or CSF
When Tested
Weekly
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9525

Human immunodeficiency virus p24 antigen

Test Method
EIA,EIA neutralisation P24 Ag
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum, Cereberospinal Fluid
Spec Type Min
500ul
When Tested
2x Weekly
Reference Ranges
HIV p24 Ag
cutoff value = neg control mean + 0.070
pos > cutoff value
neg < cutoiff value
equiv +/- 10% cutoff value
HIV p24 Ag (neutralisation)
neutralised; neg <50% inhibition
pos > 50% inhibition
Units of Measurement
optical density, S/CO
Contact
9342 9647 / 9342 9648

Human immunodeficiency virus RNA Quantitation (Virus Load, HIV-1)

Test Method
Abbott Real-Time HIV-1 assay
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
350ul plasma, CSF & Serum.
When Tested
1x Weekly
Turnaround Time
6 Days
Reference Ranges
150-10,000000 copies/ml
Cut Off Lower
Target <150 RNA copies/ml can be detected but is not quantified.
Units of Measurement
HIV-1 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625

Human immunodeficiency virus RNA Quantitation (Virus Load, HIV-1) – Ultrasensitive

Test Method
Roche COBAS 6800
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Cerebrospinal Fluid, Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
1000ul plasma, CSF & Serum.
When Tested
Daily
Turnaround Time
4 days
Reference Ranges
20-10,000000 copies/ml
Cut Off Lower
Target <20 RNA copies/ml can be detected by is not quantified
Units of Measurement
HIV-1 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625

Human immunodeficiency virus Serology Confirmation

Test Method
Western Blot IgG
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum, Cereberospinal Fluid
When Tested
3x weekly
Reference Ranges
neg=no bands or reactivity to p17 only, no other bands, pos= 2 env bands (gp160 and /or gp41 and gp120) and GAG (p17, p24, p55) or POL (p31, p51, p66), Ind= Reactivity to any viral specific band (except p17) but does not meet the positive criteria.
Contact
9342 9647 / 9342 9648

Human immunodeficiency virus Serology Screening

Test Method
Antigen/Antibody CLIA
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum, Cereberospinal Fluid
Spec Type Min
500ul
When Tested
Daily
Turnaround Time
up to 3 days
Reference Ranges
CLIA
XL S/co >= 1.0 pos :
neg pos > cutoff
.
Units of Measurement
S/CO
Contact
9342 9647 / 9342 9648

Human immunodeficiency virus Serology Supplementary

Test Method
Antigen/Antibody EIA / Manual
Specimen Required
Serum, CSF, Cadaver, Plasma, EDTA , Citrate
Spec Type Min
500ul
When Tested
3 x Weekly
Turnaround Time
up to 3 days
Reference Ranges
EIA : > cutoff = pos, equiv + or - 10% of cutoff, cutoff = neg
Units of Measurement
OD
Contact
9342 9647 / 9342 9648

Human Metapneumovirus(see Respiratory PCR)

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On Request
Reference Ranges
3-45 Ct
Contact
9342 9628

Human T lymphotropic viruses I and II Serology

Test Method
Total Antibody
Specimen Required
Blood (EDTA), Blood (Gel Tube), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Sodium Citrate), Serum
Spec Type Min
300ul
When Tested
Weekly
Reference Ranges
cutoff value = neg control mean + 0.2
pos >cutoff value
neg < cutogg value
equiv +/- 10% cutoff value
Related Tests
see under Human T lymphotropic viruses
Contact
9342 9647 / 9342 9648

Human T-Cell Lymphotrophic Virus-1 Proviral DNA Load

Test Method
Real-Time Taqman Based PCR
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Buffy Coat
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
5 days
Reference Ranges
>100 copies/million cells
Cut Off Lower
100 copies per 10^6 cells
Units of Measurement
copies per 10^6
Billing Advice
Non-rebatable
Contact
9342 9625

Human T-lymphotropic virus I and II PCR

Test Method
Real time Taqman based PCR
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Whole)
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
5 days
Reference Ranges
n/a
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625